文献情報 バイカリンは、TRIM25 を標的とすることで B 型肝炎ウイルスの複製を阻害します。
文献情報 バイカリンは、TRIM25 を標的とすることで B 型肝炎ウイルスの複製を阻害します。
この文献は2023年11月13日の文献です。 バイカリンはオウゴンの主要成分です。めちゃ最新です。
TRIM25とは何か？
これはAIではこう説明されます。
- Journal of traditional and complementary medicine. 2023 Nov;13(6);561-567. doi: 10.1016/j.jtcme.2023.05.009.
- Author
- Abstract
-
OBJECTIVE : Baicalin, which is a key bioactive constituent obtained from Scutellaria baicalensis, has been utilized in traditional Chinese medicine for many centuries. Although it has been reported that Baicalin (BA) can inhibit the replication of the Hepatitis B virus (HBV), the exact mechanism behind this process remains unclear. Interferon-stimulated genes (ISGs) are crucial in the process of antiviral defense. We aim to investigate whether BA can regulate the expression of ISGs, and thereby potentially modulate the replication of HBV.
METHODS : The study involved the use of CRISPR/Cas9 technology to perform knockout experiments on TRIM25 and IFIT3 genes. The expression of these genes was confirmed through techniques such as immunoblotting or Q-PCR. The levels of HBsAg and HBeAg were measured using ELISA, and the expression of interferon-stimulated genes was detected using a luciferase assay.
RESULTS : It is interesting to note that several ISGs belonging to the TRIM family, including TRIM5, TRIM25, and TRIM14, were induced after BA treatment. On the other hand, members of the IFIT family were reduced by BA stimulation. Additionally, BA-mediated HBV inhibition was found to be significantly restored in HepG2 cells where TRIM25 was knocked out. Additional research into the mechanism of action of BA found that prolonged treatment with BA activated the JAK/STAT signaling pathway while simultaneously inhibiting the NF-kB pathway.
CONCLUSION : The findings of our study indicate that TRIM25 has a significant impact on the regulation of HBV replication following BA treatment, providing additional insight into the mechanisms by which BA exerts its antiviral effects